The alpine newt (Ichthyosaura alpestris) is an invasive species that poses a risk to the great crested newt (Triturus cristatus) in the UK. Early detection of invasive species is key to controlling populations and environmental DNA methods provide the opportunity detect rare species without using traditional monitoring methods. This project developed a single species assay for the targeted detection of alpine newt DNA from environmental samples. The designed assay consists of primer and probe sequences for detection using quantitative PCR. The assay was tested on environmental samples with positive detection in six out of seven ponds where alpine newt had been confirmed through visual monitoring.